首页|Pan-retinal ganglion cell markers in mice,rats,and rhesus macaques

Pan-retinal ganglion cell markers in mice,rats,and rhesus macaques

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Univocal identification of retinal ganglion cells(RGCs)is an essential prerequisite for studying their degeneration and neuroprotection.Before the advent of phenotypic markers,RGCs were normally identified using retrograde tracing of retinorecipient areas.This is an invasive technique,and its use is precluded in higher mammals such as monkeys.In the past decade,several RGC markers have been described.Here,we reviewed and analyzed the specificity of nine markers used to identify all or most RGCs,i.e.,pan-RGC markers,in rats,mice,and macaques.The best markers in the three species in terms of specificity,proportion of RGCs labeled,and indicators of viability were BRN3A,expressed by vision-forming RGCs,and RBPMS,expressed by vision-and non-vision-forming RGCs.NEUN,often used to identify RGCs,was expressed by non-RGCs in the ganglion cell layer,and therefore was not RGC-specific.γ-SYN,TUJ1,and NF-L labeled the RGC axons,which impaired the detection of their somas in the central retina but would be good for studying RGC morphology.In rats,TUJ1 and NF-L were also expressed by non-RGCs.BM88,ERRβ,and PGP9.5 are rarely used as markers,but they identified most RGCs in the rats and macaques and ERRβ in mice.However,PGP9.5 was also expressed by non-RGCs in rats and macaques and BM88 and ERRβ were not suitable markers of viability.

RGCOptic nerve crushBM88BRN3AEstrogen-related receptor βERRβNEUNNeurofilament-LPGP9.5RBPMSγ-SYNβⅢ-tubulinTUJ1

Francisco M.Nadal-Nicolás、Caridad Galindo-Romero、Fernando Lucas-Ruiz、Nicholas Marsh-Amstrong、Wei Li、Manuel Vidal-Sanz、Marta Agudo-Barriuso

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Grupo de Oftalmología Experimental,Instituto Murciano de Investigación Biosanitaria Pascual Parrilla(IMIB),Murcia 30120,Spain

Dpto.Oftalmología,Facultad de Medicina,Universidad de Murcia,Murcia 30120,Spain

Retinal Neurophysiology Section,National Eye Institute,National Institutes of Health,Bethesda,Maryland 20892-2510,USA

Department of Ophthalmology and Vision Science,University of California,Davis,CA 95817,USA

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Spanish Ministry of Economy and CompetitivenessInstituto de Salud Carlos Ⅲ,Fondo Europeo de Desarrollo Regional Una manera de hacer EuropaFundación Séneca,Agencia de Ciencia y Tecnología Región de MurciaSpanish Ministry of Science and InnovationIntramural Research Program of the National Eye Institute,National Institutes of Health

PID2019-106498GB-I0PI19/0007119881/GERM/15PID 2019-106498 GB-I00NIH/NEI RO1 EY029087

2023

10.24272/j.issn.2095-8137.2022.308

动物学研究
中国科学院昆明动物研究所 中国动物学会

动物学研究

CSTPCDCSCD
影响因子：0.582
ISSN：0254-5853
年,卷(期)：2023.44(1)
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