首页|Transfer of the zp3a gene results in changes in egg adhesiveness and buoyancy in transgenic zebrafish

Transfer of the zp3a gene results in changes in egg adhesiveness and buoyancy in transgenic zebrafish

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Reproductive strategies and spawning habits play key roles in the evolution of endemic East Asian cyprinids.However,the molecular mechanisms underlying the regulation of spawning habits are not well understood.We recently identified zona pellucida(Zp)as the top differentially expressed protein between East Asian cyprinids that produce adhesive and semi-buoyant eggs,suggesting that Zp protein may play important roles in the regulation of egg type.In this work,we generated transgenic zebrafish in which oocyte-specific expression of zp genes from rare minnow(Gobiocypris rarus),an East Asian cyprinid laying adhesive eggs,was driven by a zebrafish zp3.2 gene promoter.We found that the transgenic eggs obtained partial adhesiveness and exhibited alteration in hydration and buoyancy.Abnormal metabolism of vitellogenin(VTG)may contribute to enhanced hydration and/or buoyancy.Our work shows that expression of the exogenous zp3a gene from an adhesive-egg producing fish is sufficient to induce changes in both egg adhesiveness and buoyancy in zebrafish,emphasizing the important role of zp genes in the regulation of spawning habits.Our results thus provide new insights into how endemic East Asian cyprinids may have adapted to the Yangtze river-lake system via changes in spawning habits.

Zona pellucida proteinAdhesiveBuoyancyHydrationEgg envelope

Yu-Qing Cao、Yong-Xin Wang、Yan Zhao、Jia Zhang、Xian He、Ping Xie、Jun Chen、Yu-Hua Sun

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Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,Hubei 430072,China

University of Chinese Academy of Sciences,Beijing 100049,China

Donghu Experimental Station of Lake Ecosystems,State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,Hubei 430072,China

Innovation Academy for Seed Design,Chinese Academy of Sciences,Beijing 100049,China

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Strategic Priority Research Program of the Chinese Academy of Sciences

XDB31000000

2023

10.24272/j.issn.2095-8137.2022.330

动物学研究
中国科学院昆明动物研究所 中国动物学会

动物学研究

CSTPCDCSCD
影响因子：0.582
ISSN：0254-5853
年,卷(期)：2023.44(2)
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