Abstract
Amyotrophic lateral sclerosis(ALS)is a devastating motoneuron disease,in which lower motoneurons lose control of skeletal muscles.Degeneration of neuromuscular junctions(NMJs)occurs at the initial stage of ALS.Dipeptide repeat proteins(DPRs)from G4C2 repeat-associated non-ATG(RAN)translation are known to cause C9orf72-associated ALS(C9-ALS).However,DPR inclusion burdens are weakly correlated with neurodegenerative areas in C9-ALS patients,indicating that DPRs may exert cell non-autonomous effects,in addition to the known intracellular pathological mechanisms.Here,we report that poly-GA,the most abundant form of DPR in C9-ALS,is released from cells.Local administration of poly-GA proteins in peripheral synaptic regions causes muscle weakness and impaired neuromuscular transmission in vivo.The NMJ structure cannot be maintained,as evidenced by the fragmentation of postsynaptic acetylcholine receptor(AChR)clusters and distortion of presynaptic nerve terminals.Mechanistic study demonstrated that extracellular poly-GA sequesters soluble Agrin ligands and inhibits Agrin-MuSK signaling.Our findings provide a novel cell non-autonomous mechanism by which poly-GA impairs NMJs in C9-ALS.Thus,targeting NMJs could be an early therapeutic intervention for C9-ALS.
基金项目
National Key Research and Development Program of China(2022YFF1000500)
National Key Research and Development Program of China(2021YFA1101100)
Zhejiang Provincial Natural Science Foundation(LZ22C110002)
National Natural Science Foundation of China(32271031)
National Natural Science Foundation of China(82230038)
National Natural Science Foundation of China(31871203)
National Natural Science Foundation of China(32071032)
维普
万方数据