首页|Genome-wide identification and spatiotemporal expression profiling of zinc finger SWIM domain-containing protein family genes

Genome-wide identification and spatiotemporal expression profiling of zinc finger SWIM domain-containing protein family genes

扫码查看
原文链接
  • 万方数据
  • 维普
The biological function of the novel zinc-finger SWIM domain-containing protein family (ZSWIM) during embryonic development remains elusive. Here, we conducted a genome-wide analysis to explore the evolutionary processes of the ZSWIM gene family members in mice, Xenopus tropicalis, zebrafish, and humans. We identified nine putative ZSWIM genes in the human and mouse genome, eight in the Xenopus genome,and five in the zebrafish genome. Based on multiple sequence alignment, three members, ZSWIM5, ZSWIM6,and ZSWIM8, demonstrated the highest homology across all four species. Using available RNA sequencing (RNA-seq) data, ZSWIM genes were found to be widely expressed across different tissues, with distinct tissue-specific properties. To identify the functions of the ZSWIM protein family during embryogenesis, we examined temporal and spatial expression patterns of zswim family genes in Xenopus embryos. Quantitative real-time polymerase chain reaction (qRT-PCR) revealed that each member had a distinct expression profile. Whole-mount in situ hybridization showed that both zswim1 and zswim3 were maternally expressed genes; zswim5 and zswim6 were expressed throughout embryogenesis and displayed dynamic expression in the brain, eyes, somite, and bronchial arch at the late tailbud stages; zswim7 was detected in the eye area; zswim8 showed a dynamic expression pattern during the tailbud stages, with expression detected in the brain, eyes, and somite;zswim9 was faintly expressed throughout embryonic development. This study provides a foundation for future research to delineate the functions of ZSWIM gene members.

ZSWIM gene familyXenopusGene expressionPhylogenetic analysisBioinformatics

Imtiaz UI Hassan、Hafiz Mamoon Rehman、Ziran Liu、Liangji Zhou、Muhammad Kaleem Samma、Chengdong Wang、Zixin Rong、Xufeng Qi、Dongqing Cai、Hui Zhao

展开 >

Key Laboratory for Regenerative Medicine,Ministry of Education,School of Biomedical Sciences,Faculty of Medicine,The Chinese University of Hong Kong,Hong Kong SAR,China

Centre for Agricultural Biochemistry and Biotechnology(CABB),University of Agriculture Faisalabad,Faisalabad 38000,Pakistan

Qingdao Municipal Center for Disease Control and Prevention,Qingdao,Shandong 266034,China

Department of Biology and Chemistry,City University of Hong Kong,Hong Kong SAR,China

Department of Gene Technology,School of Engineering Sciences in Chemistry,Biotechnology and Health(CBH),KTH Royal Institute of Technology,10691 Stockholm,Sweden

Key Laboratory of Regenerative Medicine of Ministry of Education,Department of Developmental & Regenerative Biology,Jinan University,Guangzhou 511436,China

Kunming Institute of Zoology-The Chinese University of Hong Kong(KIZ-CUHK)Joint Laboratory of Bioresources and Molecular Research of Common Diseases,Hong Kong SAR,China

Hong Kong Branch of CAS Center for Excellence in Animal Evolution and Genetics,The Chinese University of Hong Kong,Hong Kong SAR,China

展开 >

National Key R&D Program of China, Synthetic Biology ResearchResearch Grants Council of Hong KongResearch Grants Council of Hong KongResearch Grants Council of Hong KongResearch Grants Council of Hong KongHong Kong Branch of CAS Center for Excellence in Animal Evolution and Genetics,Chinese University of Hong Kong

2019YFA09045001411912014112618CRF C5033-19E

2023

10.24272/j.issn.2095-8137.2022.418

动物学研究
中国科学院昆明动物研究所 中国动物学会

动物学研究

CSTPCDCSCD
影响因子：0.582
ISSN：0254-5853
年,卷(期)：2023.44(3)
  • 36