首页|Cross-species recognition of bat coronavirus RsYN04 and cross-reaction of SARS-CoV-2 antibodies against the virus

Cross-species recognition of bat coronavirus RsYN04 and cross-reaction of SARS-CoV-2 antibodies against the virus

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  • 国家科技期刊平台
  • NETL
  • NSTL
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Following the outbreak of coronavirus disease 2019(COVID-19),several severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)-related coronaviruses have been discovered.Previous research has identified a novel lineage of SARS-CoV-2-related CoVs in bats,including RsYN04,which recognizes human angiotensin-converting enzyme 2(ACE2)and thus poses a potential threat to humans.Here,we screened the binding of the RsYN04 receptor-binding domain(RBD)to ACE2 orthologs from 52 animal species and found that the virus showed a narrower ACE2-binding spectrum than SARS-CoV-2.However,the presence of the T484W mutation in the RsYN04 RBD broadened its range.We also evaluated 44 SARS-CoV-2 antibodies targeting seven epitope communities in the SARS-CoV-2 RBD,together with serum obtained from COVID-19 convalescents and vaccinees,to determine their cross-reaction against RsYN04.Results showed that no antibodies,except for the RBD-6 and RBD-7 classes,bound to the RsYN04 RBD,indicating substantial immune differences from SARS-CoV-2.Furthermore,the structure of the RsYN04 RBD in complex with cross-reactive antibody S43 in RBD-7 revealed a potently broad epitope for the development of therapeutics and vaccines.Our findings suggest RsYN04 and other viruses belonging to the same clade have the potential to infect several species,including humans,highlighting the necessity for viral surveillance and development of broad anti-coronavirus countermeasures.

SARS-CoV-2-related coronavirusRsYN04ACE2AntibodyStructure

Runchu Zhao、Sheng Niu、Pu Han、Yue Gao、Dezhi Liu、Chunliang Luo、Honghui Liu、Bo Liu、Yanli Xu、Jianxun Qi、Zhihai Chen、Weifeng Shi、Lili Wu、George F.Gao、Qihui Wang

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Institute of Physical Science and Information,Anhui University,Hefei,Anhui 230039,China

CAS Key Laboratory of Pathogen Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China

College of Veterinary Medicine,Shanxi Agricultural University,Jinzhong,Shanxi 030801,China

School of Life Sciences,Hebei University,Baoding,Hebei 071002,China

Center of Infectious Disease,Beijing Ditan Hospital,Capital Medical University,Beijing 100015,China

Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases in Universities of Shandong,Shandong First Medical University & Shandong Academy of Medical Sciences,Taian,Shandong 271000,China

University of Chinese Academy of Sciences,Beijing 100049,China

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National Key R&D Program of ChinaNational Natural Science Foundation of ChinaChinese Academy of SciencesChinese Academy of Sciences

2022YFC230340382225021YSBR-010Y2022037

2023

10.24272/j.issn.2095-8137.2023.187

动物学研究
中国科学院昆明动物研究所 中国动物学会

动物学研究

CSTPCDCSCD
影响因子：0.582
ISSN：0254-5853
年,卷(期)：2023.44(6)
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