Cross-species recognition of bat coronavirus RsYN04 and cross-reaction of SARS-CoV-2 antibodies against the virus

Runchu Zhao ¹Sheng Niu ²Pu Han ³Yue Gao ⁴Dezhi Liu ¹Chunliang Luo ⁵Honghui Liu ³Bo Liu ⁵Yanli Xu ⁶Jianxun Qi ³Zhihai Chen ⁶Weifeng Shi ⁷Lili Wu ³George F.Gao ⁸Qihui Wang⁹

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作者信息

1. Institute of Physical Science and Information,Anhui University,Hefei,Anhui 230039,China;CAS Key Laboratory of Pathogen Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China
2. College of Veterinary Medicine,Shanxi Agricultural University,Jinzhong,Shanxi 030801,China
3. CAS Key Laboratory of Pathogen Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China
4. CAS Key Laboratory of Pathogen Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;School of Life Sciences,Hebei University,Baoding,Hebei 071002,China
5. CAS Key Laboratory of Pathogen Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;College of Veterinary Medicine,Shanxi Agricultural University,Jinzhong,Shanxi 030801,China
6. Center of Infectious Disease,Beijing Ditan Hospital,Capital Medical University,Beijing 100015,China
7. Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases in Universities of Shandong,Shandong First Medical University & Shandong Academy of Medical Sciences,Taian,Shandong 271000,China
8. CAS Key Laboratory of Pathogen Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;University of Chinese Academy of Sciences,Beijing 100049,China
9. Institute of Physical Science and Information,Anhui University,Hefei,Anhui 230039,China;CAS Key Laboratory of Pathogen Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;University of Chinese Academy of Sciences,Beijing 100049,China
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Abstract

Following the outbreak of coronavirus disease 2019(COVID-19),several severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)-related coronaviruses have been discovered.Previous research has identified a novel lineage of SARS-CoV-2-related CoVs in bats,including RsYN04,which recognizes human angiotensin-converting enzyme 2(ACE2)and thus poses a potential threat to humans.Here,we screened the binding of the RsYN04 receptor-binding domain(RBD)to ACE2 orthologs from 52 animal species and found that the virus showed a narrower ACE2-binding spectrum than SARS-CoV-2.However,the presence of the T484W mutation in the RsYN04 RBD broadened its range.We also evaluated 44 SARS-CoV-2 antibodies targeting seven epitope communities in the SARS-CoV-2 RBD,together with serum obtained from COVID-19 convalescents and vaccinees,to determine their cross-reaction against RsYN04.Results showed that no antibodies,except for the RBD-6 and RBD-7 classes,bound to the RsYN04 RBD,indicating substantial immune differences from SARS-CoV-2.Furthermore,the structure of the RsYN04 RBD in complex with cross-reactive antibody S43 in RBD-7 revealed a potently broad epitope for the development of therapeutics and vaccines.Our findings suggest RsYN04 and other viruses belonging to the same clade have the potential to infect several species,including humans,highlighting the necessity for viral surveillance and development of broad anti-coronavirus countermeasures.

Key words

SARS-CoV-2-related coronavirus/RsYN04/ACE2/Antibody/Structure

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基金项目

National Key R&D Program of China(2022YFC2303403)

National Natural Science Foundation of China(82225021)

Chinese Academy of Sciences(YSBR-010)

Chinese Academy of Sciences(Y2022037)

出版年

2023

动物学研究

中国科学院昆明动物研究所中国动物学会

动物学研究

CSTPCDCSCD

影响因子：0.582

ISSN：0254-5853

参考文献量1

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