Experimental Study on the Display of Muscle and Nerve Tissue of Aspidogaster conchicola by Immunofluorescence
[Objectives]The study aims tounderstand the regulation of the Aspidogaster conchicola nervous system on muscles.[Methods]The naturally infected Aspidogaster conchicola were collected from the pericardium ofAnodonta woodiana.According to the development degree of the testis,ovary,uterus,vitelline glands,and other reproductive organs in the insect body under the microscope,the insect body was divided into larva group and adult group.After being fixed with 4％formalin,the muscle fibers and nerve fibers of the larva and adult were observed by phalloidin staining and acetylated tubulin immunohistochemistry,respectively,and were observed by confocal laser microscopy.[Results]Through phalloidin fluorescent staining,we found that the muscle tissue of adult was more developed than that of larvae,and there are two different types of muscle fibers distributed in the body wall,the outer layer is longitudinal muscle fibers,the inner layer is annular muscle fibers,and the muscle fibers are reticulate(Fig.2a),and the muscle fibers of oral sucker(Fig.2b),uterus and cirrus pouch(Fig.2f),and adhesive disc(Fig.2g)was more densely distributed.Fluorescence staining of acetylated tubulin showed that the neural network of adults was more complex than that of larvae,and some nerve fibers of reproductive organs could be seen(Fig.4b,c).The results of double staining showed that the distribution of muscle fibers and nerve fibers on different organs of the fluke were consistent with each other(Fig.5).[Conclusion]The trematode may regulate its muscle activity through the neural network.
万方数据
维普
