The Spatial-Temporal Distribution of Cytokines in the Wound Healing Stage of Regenerating Tail in Scincella tsinlingensis
[Objectives]Cytokines participate in the immune response,mediate the inflammatory response,and then play a regulatory role in the regeneration and repair process of damaged tissues.[Methods]The tail of Scincella tsinlingensis was amputated with a razor blade to set up tail regeneration model,and immunohistochemical methods were used to detect the histologic localization and expression changes of cytokines interleukin,IL-6,8,1β(IL-6,IL-8,IL-1β),interleukin 1 receptor type 1(IL-1R1),tumour necrosis factor-α(TNF-α)and interleukin,IL-10(IL-10)at the stage of wound healing.Three visual fields of different parts were randomly selected under microscope with 400-fold magnification for immunopositive cell count.The results were expressed as"Mean±SD",one-way ANOVA and Tukey HSD were used to analyze the difference in the number of positive cells on different days.[Results]The results showed that six cytokines were positive in bone cells in the spinal cord and vertebrae.The number of IL-6 positive cells in the wound site and the dermis of proximal scale to the stump surface initially showed increasing and later decreasing of these cells,reaching a peak of 194.3±24.9 at 3 d after tail amputation,the number of IL-8 positive cells in the wound surface was 107.7±12.7,exceeding 43.7±9.1 in the dermis at 3 d after tail amputation.The number of IL-1β and IL-1R1 positive cells initially showed increasing and later decreasing of these cells,and significantly more positive cells occurred in the wound site than in the dermis(P＜0.05).The TNF-α positive cells increased from 126.3±35.0 at 0.5 d to 190.3±12.1 at 3 d.The number of anti-inflammatory cytokine IL-10 positive cells initially showed increasing and later decreasing of these cells after tail amputation.At 0.5 d after tail amputation,positive cells reached 201±17.8,which is significantly higher than that in the original tail(P＜0.05,Fig.7).[Conclusion]The above results indicate that IL-6,IL-8,IL-1β,IL-1R1,TNF-αand IL-10 are consistent with the spatial distribution of myeloid-derived cells,jointly participate in tail wound healing of S.tsinlingensis,and may achieve scarless wound healing and promote blastema formation by maintaining an immuno-suppressive microenvironment.
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