Achalinus yunkaiensis and A.huangjietangi Found in Lucheng,Zhejiang,China
Two specimens of genus Achalinus(2022AZJLCN1 and 2023AZJLCN2)were collected from Yangyi Reservoir,Lucheng District,Wenzhou City,Zhejiang Province in September 2022 and April 2023.They were similar to A.yunkaiensis and A.huangjietangi in morphology,respectively.The results of the maximum likelihood tree based on COI gene sequences showed that they were clustered into a clade with these two species respectively.The p-distance of 2023AZJCN2 and A.yunkaiensis was 0.10,lower than that between Achalinus species ranging from 0.12 to 0.16 in China.The p-distance of 2022AZJLCN1 and A.huangjietangi was 0.09,which is not higher than that between A.huangjietangi ranging from 0.04 to 0.12.In the end,2023AZJLCN2 and 2022AZJLCN1 were identified as A.yunkaiensis and A.huangjietangi.The previous record of A.yunkaiensis was found in Guangdong and Guangxi of China,the discovery in Lucheng District is a new record in Zhejiang Province.And A.huangjietangi was distributed in Anhui and Zhejiang Provinces,the discovery in Lucheng District is the first new record of its distribution in Wenzhou City.The new records of those two species suggested a wider distribution range.
Mitochondrial COI geneMaximum likelihood treeGenetic distanceNew record
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