摘要
2023年10月,在广西百色市右江区采集到无尾目(Anura)树蛙科(Rhacophoridae)棱皮树蛙属(Theloderma)物种成体雄性和亚成体标本各1号,经形态特征比较,所采集标本与印支棱皮树蛙(T.gordoni)基本一致.基于16S rRNA基因563 bp片段的系统发育分析显示,本次采集的2号标本(共享单倍型)与印支棱皮树蛙聚为一支,遗传距离为0.7％～4.0％,小于与属内其他物种间遗传距离(9.3％～15.3％).综合形态特征和分子系统发育比较,确定此次采集到的2号标本为印支棱皮树蛙,为广西两栖动物分布新记录种.
Abstract
Theloderma gordoni was described from Chiang Mai,Thailand by Taylor,1962.In 2018,it was confirmed to have a distribution in Yunnan Province,China,which is the first record from China.During a field survey in October 2023,one adult male and one subadult specimen of genus Theloderma were collected from Youjiang District,Baise City,Guangxi Zhuang Autonomous Region,China(23°44′07″ N,106°23′43″ E;780 m a.s.1.).Through morphological comparison,the newly collected specimens were basically consistent with the morphological characteristics of T gordoni(Fig.1).Based on 16S ribosomal RNA(16S rRNA)gene fragment,we performed maximum likelihood phylogenetic analyses using IQ-TREE 1.6.12 and calculated the uncorrected genetic distances within Theloderma using MEGA X.All the new sequences in this study have been deposited in GenBank,and the other sequences used were retrieved from GenBank(Table 1).According to the 16S rRNA phylogenetic analysis revealed that the specimens and T.gordoni specimens clustered together(Fig.2),with uncorrected pairwise distances(p-distance)from 0.7％to 4.0％(Table 3),which are less than the p-distances between species of the genus Theloderma(9.3％-15.3％).Based on the morphological and molecular phylogenetic analysis results,the two specimens were identified as T.gordoni(Anura,Rhacophoridae),making a new record of amphibians for the Guangxi Zhuang Autonomous Region.
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