Theloderma gordoni was described from Chiang Mai,Thailand by Taylor,1962.In 2018,it was confirmed to have a distribution in Yunnan Province,China,which is the first record from China.During a field survey in October 2023,one adult male and one subadult specimen of genus Theloderma were collected from Youjiang District,Baise City,Guangxi Zhuang Autonomous Region,China(23°44′07″ N,106°23′43″ E;780 m a.s.1.).Through morphological comparison,the newly collected specimens were basically consistent with the morphological characteristics of T gordoni(Fig.1).Based on 16S ribosomal RNA(16S rRNA)gene fragment,we performed maximum likelihood phylogenetic analyses using IQ-TREE 1.6.12 and calculated the uncorrected genetic distances within Theloderma using MEGA X.All the new sequences in this study have been deposited in GenBank,and the other sequences used were retrieved from GenBank(Table 1).According to the 16S rRNA phylogenetic analysis revealed that the specimens and T.gordoni specimens clustered together(Fig.2),with uncorrected pairwise distances(p-distance)from 0.7％to 4.0％(Table 3),which are less than the p-distances between species of the genus Theloderma(9.3％-15.3％).Based on the morphological and molecular phylogenetic analysis results,the two specimens were identified as T.gordoni(Anura,Rhacophoridae),making a new record of amphibians for the Guangxi Zhuang Autonomous Region.
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