Regional holographic theory applies holographic cognitive principles to regional studies,and it is a deconstruction of regional research with a philosophical framework.The core idea is that the region is a multi-level,multi-dimensional and bounded holographic system composed of countless interconnected holographic elements.Each holographic element is the epitome of the whole region,and the correlation between holographic elements drives the evolution of the region.The theory dialectically treats the integrity and partiality of the region,unifies the uniqueness,integrity and relevance of the region from the perspective of information,and provides a new theoretical perspective for regional research.Based on holographic theory and regional research,this paper systematically sorts out the concept,structure and relationship of holographic region,as well as the theoretical connotation and applica-tion paradigm of regional holographic theory,and carries out application practice in four aspects:holographic geo-graphic database,regional holographic integrated development strategy,regional holographic coordinated develop-ment model and acupuncture treatment of diseases in big cities,with a view to providing theoretical reference for re-gional coordinated development in the new period.