Microbial Diversity in the Hypersaline Dabuxun Lake in Qaidam Basin, China
Microbial diversity in the water and sediment of the hypersaline Dabuxun Lake was investigated by using culture-dependent and-independent techniques including cultivation,quantitative polymerase chain reaction (qPCR),and 16S rRNA gene fragment 454 pyrosequencing.The qPCR results showed that in the water of the Dabuxun Lake the bacterial and archaeal 16S rRNA gene abundances were 3.27 × 107 and 4.35 × 104 copies/mL,respectively.The cultivation-based phylogenetic analysis showed that bacterial isolates were classified as Pseudomonas and Bacillus,and archaeal isolates were affiliated with three haloarchaeal genera:Haloarcula,Halorubrum,and Halorhabdus.The 454 pyrosequencing-based phylogenetic results showed that the major bacterial phyla were Actinobacteria,Bacteroidetes,Proteobacteria,and Verrucomicrobia,with each accounting for 53.0％,25.8％,14.1％ and 1.6％ of the total sequence reads,respectively.The dominant archaeal reads belong to Halobacteriaceae,accounting for 5.5 ％ of total reads.Our results gave a thorough understanding of the microbial composition in the hypersaline Dabuxun Lake.
NETL
NSTL
万方数据
维普
