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镉胁迫对忍冬抗氧化酶活性及内源激素含量的影响

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为研究Cd胁迫下忍冬(Lonicera japonica Thunb.)抗氧化酶活性和内源激素水平的响应变化,采用土培试验,设置不同Cd处理(0、2.5、5、10、25、50和100 mg/kg),分析植株生物量、叶片丙二醛(MDA)含量、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)活性以及生长素(IAA)、赤霉素(GA,)、脱落酸(ABA)的含量.结果表明:2.5 mg/kg Cd处理时地下部生物量增加20.93%,超过25 mg/kg Cd处理时地下部、地上部生物量均显著下降,最多降低了 28.78%和40.92%.在各Cd处理下,叶片MDA含量没有受到显著影响;SOD和POD活性显著增强,CAT活性先升高后降低,APX活性没有受到显著影响.叶片IAA、GA3含量、GA3/ABA和IAA/ABA先升高后降低;ABA含量升高,比对照最多增加55.88%.相关性分析表明,忍冬叶片中抗氧化酶SOD、POD活性与GA3含量存在显著负相关性,与ABA含量存在显著正相关性;而CAT活性则与IAA和GA3含量存在显著正相关性,与ABA存在显著负相关性.忍冬对Cd的耐受能力很强,在各Cd胁迫处理下没有受到明显的过氧化损伤,其较强的抗氧化酶活性和大量累积的ABA可能是重要保护机制.
Effect of Cadmium Stress on Antioxidant Enzyme Activity and Endogenous Hormones Content in Lonicera japonica Thunb.
In order to study the response change of Lonicera japonica Thunb.to cadmium(Cd)stress antioxidant enzyme activity and endogenous hormone levels,the plant biomass,malondialdehyde(MDA)content,the activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),ascorbic acid peroxidase(APX),and the content of auxin(IAA),gibberellin(GA3),abscisic acid(ABA)in leaves was analyzed by pot experiments with various Cd concentrations(0、2.5、5、10、25、50 and 100 mg/kg).The results showed that the underground biomass increased by 20.93%at low concentrations of 2.5 mg/kg Cd treatment,while above 25 mg/kg Cd,both underground and above-ground biomass significantly decreased,with a maximum reduction of 28.78%and 40.92%.With increasing Cd treatment concentrations,the MDA content in leaves was not significantly affected,while the activities of SOD and POD were significantly enhanced,CAT activity first increased and then decreased,APX activity was not significantly affected.The content of IAA,GA3,GA3/ABA and IAA/ABA in leaves were firstly increased and then decreased.The ABA content increased by 55.88%compared with the control.Correlation analysis showed that the activities of SOD and POD were negatively correlated with GA3 content,but positively correlated with ABA.The activities of CAT and APX were positively correlated with IAA and GA3 content,but negatively correlated with ABA.Taken together,the results indicated that L.japonica had a strong tolerance to Cd,and the plant had not suffered significant oxidative damage under different level of Cd stress.Its strong antioxidant enzyme activity and a large amount of accumulated ABA may be important protective mechanisms.

Lonicera japonica Thunb.Cd stressantioxidant enzymesendogenous hormones

贾莲、张冬、张吉斯、吕琳琳、刁全平

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鞍山师范学院化学与生命科学学院,辽宁省天然产物活性分子开发及利用重点实验室,辽宁鞍山 114007

北方测盟测试技术(辽宁)有限公司,沈阳 110042

忍冬 镉胁迫 抗氧化酶 内源激素

辽宁省自然科学基金辽宁省教育厅青年扶持项目鞍山师范学院大学生创新创业训练计划

2019-MS-001jytqnfc202103202110169025

2024

地球与环境
中国科学院地球化学研究所

地球与环境

CSTPCD北大核心
影响因子:0.875
ISSN:1672-9250
年,卷(期):2024.52(1)
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