Prediction of Lead,Zinc and Cadmium Concentrations in Soil Based on the Response of Chlorella pyrenoidosa
Prediction of heavy metal concentration in soil based on green algae response has the advantages of short time and low cost.In this study,Pb,Zn,Cd and their mixture contaminated soil were taken as the research object,and Chlorella pyrenoidosa was taken as the test organism.The concentration of single heavy metal and its mixture was predicted by the response of soil extract exposure.The results showed that the soil extracts polluted by different concentrations of Pb,Zn,Cd and their mixtures had smooth and monotonous concentration-response curves(CRCs)for Chlorella pyrenoidosa;The concentration of heavy metals and their binary and ternary mix-tures is in direct proportion to the growth inhibition rate of Chlorella pyrenoidosa when the exposure time is 96-168 h.The single and mixture heavy metal contaminated soil extract that did not test the response of green algae were exposed to green algae.Each heavy metal and its mixture were set with 2 to 3 concentration points to test its toxicity response to Chlorella pyrenoidosa.The toxicity response values were substituted into the CRCs of single heavy metal and its mixture to predict the concentration of heavy metal and its mixture.The results showed that 96h exposure data could accurately predict soil Pb,Zn,Cd single and mixed pollution.Therefore,it is feasible to predict the concentration of Pb,Zn and Cd in soil based on the response of Chlorella pyrenoidosa,and the 96 h exposure data of green algae is more suitable for prediction.
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