Effect of renal ischemia-reperfusion injury on ultrastructure of glomerular filtration barrier in rats
This study investigated the ultrastructural changes in the glomerular filtration barrier(GFB)induced by renal ischemia-reperfusion injury(IRI)in rats.Twelve male SD rats were randomly divided into two groups:The Sham group(Sham group)and the Ischemia-reperfusion injury group(IRI group)with six rats in each group.Ultrastructural changes in the GFB were examined by transmission electron microscopy and a lanthanum nitrate tracer technique.In the Sham group,the structure of the GFB was normal,including the fenestrated endothelium,the intervening glomerular basement membrane(GBM),and the podocyte slit diaphragm.The fenestrated endothelium sticked closely to the inner side of the intervening GBM.The podocyte foot processes were arranged in a palisading pattern,adhering to the outside of the GBM.The slit diaphragm between the foot processes was clearly visible.In the IRI group,extensive effacement of the foot processes was observed.The slit diaphragm between foot processes disappeared,replaced by occluding junctions.Many microvilli were observed on the surface of podocytes.Partial GBM was irregular and thickened,displaying separated layer and rupture.The podocyte foot processes detached from the GBM,leaving parts of the GBM exposed and compromising the integrity of the GFB.Lanthanum nitrate tracer showed that many lanthanite particles deposited in the fenestra of endothelial cell and the lamina rarainterna of the GBM in the Sham group,with few lanthanite particles crossing the lamina densa and lamina raraexterna of GBM into Bowman's space.In the IRI group,many lanthanite particles deposited in the endothelial cell fenestra and three layers of the GBM,with some lanthanite particles entering Bowman's space.These findings showed that IRI disrupted the integrity of the GFB and increased its permeability.
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