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灰树花子实体转录组测序和分析

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本研究首次构建了灰树花子实体高质量cDNA文库,并利用Illumina高通量测序技术对其转录组进行了测序,采用生物信息学方法开展基因表达谱的研究、功能基因的预测.序列拼接共获得63 137个Unigene,通过BLAST比对NR数据库,获得46 670个被注释的Unigene.根据KEGG pathway数据库,对灰树花转录组的Unigene进行了Pathway生物学通路的注释和预测,共有27 472个Unigene被注释,被Unigene注释到的Pathway有239个.SSR查找发现,从6 3 137个UniGene中共查找到5 294个SSR位点,占Unigene总数的比例为8.38%.其中,单核苷酸重复所占比例最高,达到63.4%,其次是三核苷酸重复,为24.44%.SSR不同重复基元类型中,出现频率最高的为A/T,其次是CCG/CGG,AG/CT.
Analysis on Transcriptome Sequenced of Maitake
The transcriptome of the fruiting body of maitake was sequenced by Illumina to study the expression profiling and predict the functional gene.63 137 Unigenes were obtained through sequence assembly,for which 46 670 Unigenes were annotated through NR database by BLAST.A total of 27 472 Unigenes were identified to correspond with 239 biological pathways by annotating and forecasting the biological pathways for the transcriptome of Maitake.There were 5 294 SSR in 63 137 Unigenes were found,which accounting for 8.38% proportion of the total number of Unigenes.The characteristic of SSR distribution showed that mono-nucleotide repeat was the highest reaching 63.4%,following by tri-nucleotide repeat,which was 24.44%.The types of SSR were analyzed that A/T was the highest repeat,following by CCG/CGG and AG/CT.

Maitaketranscriptome sequenceunigenefruit body

袁卫东、陆娜、陈青、宋吉玲、王伟科

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杭州市农业科学研究院,杭州310024

浙江省农业技术推广中心,杭州310020

灰树花 转录组测序 Unigene 子实体

杭州市珍稀食用菌科技创新服务平台

20072612Y09

2015

复旦学报(自然科学版)
复旦大学

复旦学报(自然科学版)

CSTPCDCSCD北大核心
影响因子:0.388
ISSN:0427-7104
年,卷(期):2015.54(5)
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