基因的剪接方式在不同发育时期、不同组织以及不同外界环境条件下可以发生改变，即可变剪接。可变剪接在转录后水平上调控基因功能，增加基因功能的复杂性和多样性。目前已发现一些基因在环境因素刺激下会发生可变剪接，但是逆境下全基因组水平上的可变剪接事件在很多植物中还未完全了解，这些事件是否同时受到植物激素的调控目前也不清楚。为了了解水稻在外界逆境胁迫下的全基因组可变剪接事件，特别是脱落酸(ABA)信号途径被激活后哪些基因发生了可变剪接，我们对盐胁迫以及 ABA处理后水稻转录组中的可变剪接事件进行了交叉分析，发现一些信号途径的基因在盐胁迫以及 ABA 处理后均发生了可变剪接。选择其中与DNA损伤修复以及 ABA 信号转导有关的基因进行了进一步分析，并通过 RT-PCR 对其中OsPP2C67 和OsSADR1 基因的可变剪接进行了验证，得到的结果与分析结果一致。
Analysis of Alternative Splicing Events in Rice Genome under Salt Stress and ABA Treatment
A single gene may generate different but related mRNA transcripts in different development stages,different tissues and different environmental conditions,which is called alternative splicing.Alternative splicing regulates gene functions at the post transcriptional level,increasing the complexity and diversity of gene functions.Although some genes have been found to undergo alternative splicing under external environmental stimuli,the genome-wide alternative splicing events have not been fully understood in many plants,and whether they are regulated by plant hormones are also not clear.In order to understand the whole genome alternative splicing events of rice under salt stress,especially when ABA signaling pathway is activated,we compared the alternative splicing events of rice transcriptome after salt stress and ABA treatment,and found that some genes underwent alternative splicing in both conditions.Genes involved in DNA damage repair and ABA signaling transduction were selected for further analysis.RT-PCR results verified the alternative splicing of OsPP2C67 and OsSADR1 under salt stress and ABA treatment.
万方数据
维普
