LEPREL1 as a Potential Therapeutic Target for Idiopathic Pulmonary Fibrosis
To find potential therapeutic target for idiopathic pulmonary fibrosis(IPF),GEO was used to get gene microarray datas,R was used to analyse differentially expressed genes and pathways;Strings and Cytoscape were used to screen HUB genes(key genes);RNA-seq and single cell data were as data validation,lung fibrosis mice were as experiment validation.The results showed that gene microarray datasets GSE10667,GSE53845,GSE48149 have 116 samples and 132 differential genes,pathways were enriched in extracellular matrix,collagen and so on.COL1A1,CXCL12,LEPREL1 and other genes were screened as HUB genes by STRING and Cytoscape.Among HUB genes,LEPREL1 expression was significantly down-regulated(GSE10667,Padj=0.000 96;GSE53845,Padj=0.000 104 8;GSE48149,Padj=0.015 17).In the RNA-Seq datasets GSE92592(Padj=0.028 8),GSE83717(Padj=0.006 268),GSE150910(Padj=1.952 8E-30)LEPREL1 were also significantly down-regulated;in the single cell data(GSE135893)LEPREL1 was mainly expressed in AT2 cells and tended to be down-regulated;it was also significantly down-regulated in RT-qPCR and 1HC in lung fibrosis mice.81 differentially expressed genes were associated with LEPREL1,mainly enriched in cell adhesion,calcium,collagen and Wnt pathways.The differential genes in IPF are mainly enriched in collagen and extracellular matrix pathways,and LEPREL1,which is down-regulated in AT2 cells,may be a potential therapeutic target for IPF.
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