Remove Olfm4+Intestinal Stem Cells in Mice Model was Constructed and Evaluate Its Effects on Mice Survival Development after Birth
The small intestine is the main organ responsible for digestion and absorption in the human body.It has a strong self-renewal ability,relying on LGR5+small intestine stem cells at the bottom of the glandular fossa.It will completely renew once in 3-7 days on average.LGR5 is the most typical marker of intestinal stem cells and olfactomedin 4(Olfm4)is a new markers of small intestinal stem cell,having different expression with the LGR5 after birth stage.In adults,when LGR5+small intestinal stem cells are damaged,+4 reserve stem cells and intestinal secretory cells can be converted into LGR5+small intestinal stem cells to supplement the loss of LGR5+small intestinal stem cells.In order to investigate whether there is a cell group that can supplement the function of LGR5+small intestinal stem cells in postnatal development when LGR5+small intestinal stem cells are not mature,transgenic mice were used to kill Olfm4+small intestinal stem cells at 9 days after birth,and the intestinal function was determined by HE staining,qPCR and immunohistochemistry.The results showed that killing Olfm4+small intestinal stem cells at postnatal stage did not affect Small intestinal structure and mouse survival,and it was speculated that Sox9 positive small intestinal progenitor cells might compensate for the function of Olfm4+small intestinal stem cells.
small intestinal stem cellspostnatal developmentOlfm4Sox9
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