The Effect of Blocking Different TGF-β Signaling Target Sites on Zebrafish Heart Development
Defects in heart development can lead to congenital heart diseases.The transforming growth factor-β(TGF-β)signaling pathway plays a crucial role in heart development.Several zebrafish reporter lines were utilized,along with immunofluorescence and in situ hybridization technology,to investigate the impact of suppressing different target sites of the TGF-β signaling pathway on cardiomyocyte(CM)proliferation,cell size and trabecular development.The results showed that blocking TGFBR1 had no effect on CM proliferation.However,suppressing TGFBR1 and TGFBR2 promoted CM proliferation and trabecular development.On the other hand,impairing Smad3 resulted in cell cycle arrest and did not affect cell size and trabecular development.These findings provide a theoretical basis for considering TGF-β as a potential intervention target for heart development defects.
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