Natural spider silk has superior mechanical properties,and its various forms have excellent antibacterial activity,biocompatibility,and good thermal conductivity.It is of great significance to develop natural biological drug-loaded materials with good biocompatibility for reducing the strong immunogenicity reaction of patients.By explor-ing the dissolution and membrane-forming methods of spider silk in different solvents,the reaction conditions were optimized,hexafluoroisopropanol was used as the dissolution solvent,the material ratio was 1∶1(mg∶mL),the spider silk was dissolved at 60℃for 8 h,and the membrane was formed by solvent casting,and the drug release in vitro of rhodamine B(RhB)as the model drug was explored with the correlation characterization.The cytotoxicity of the material was tested by extraction method.The spider fibroin membrane extract co-culture with cells showed that the material had no potential cytotoxicity to mouse embryonic osteoblasts cells.The drug-loaded spider silk fibroin mem-brane prepared by this method has high yield,uniform thickness and simple operation,which provides a simple and feasible technology for the dissolution of natural spider silk protein and the preparation of membrane agent.
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