The twisted bamboo fiber(TBF)was used as the reinforcement phase and the epoxy resin-anhydride sys-tem as the matrix phase to prepare the twisted bamboo fiber/epoxy resin(TBF/EP)composite.The effects of alkali treatment on the wettability and tensile failure of TBF/EP composites were investigated by varying the concentra-tion of NaOH solution(1wt%-5wt%).Nano-scale and micro-scale experimental techniques,such as SEM,surface tension testing,and in-situ loading,were employed to analyze the fiber-resin interface,wetting properties,and tensile mechanical properties of the composites.The results show that alkali treatment reduces the surface energy and polarity of the fibers,resulting in a decrease in the wetting force between TBF and the matrix from 0.45 mN to 0.1 mN.The TBF/EP composite modified with 3wt%NaOH solution exhibits a tensile strength(TS)of 273.70 MPa,which is 178.64%higher than that of the untreated composite.In-situ analysis reveals that the failure process of TBF involves fiber fracture and fiber sliding,while the failure process of the TBF/EP composite includes matrix shear yielding and fiber fracture.Moreover,as the wetting properties improve,the inhibitory effect of the fibers on matrix yielding increase.Therefore,the strength of the TBF/EP composite is mainly derived from the reinforcement of the fibers and the interface,which is influenced by wetting properties and stress transfer effect between TBF and matrix.