Ag量子点协同四环素的抑菌及其机制
Bacteriostatic performance and mechanism of Ag quantum dots synergistic tetracycline
郭少波 1陈惠惠 2刘轲 3胡瑞玲 2王嘉伟 2余凡 4刘智峰 1史娟 1郭婷 1季晓晖 1张田雷1
作者信息
- 1. 陕西理工大学 化学与环境科学学院,汉中 723000;陕西省催化基础与应用重点实验室,汉中 723000
- 2. 陕西理工大学 化学与环境科学学院,汉中 723000
- 3. 延安市食品质量安全检测检测中心,延安 716000
- 4. 城固县市场监督管理局,汉中 723000
- 折叠
摘要
四环素类抗生素因具有高效、低毒、广谱抑菌性等优点而被广泛使用,但随着抗生素的滥用致使大量的耐药菌出现,使四环素类抗生素的药用价值逐渐降低.超小粒径的纳米Ag虽可使细菌甚至耐药菌失活,但单独使用毒性较强,且易团聚.为此,本文利用Ag的d轨道为满电子结构,可与供电子基团配位的原理,设计了核壳型介孔Fe3O4@SiO2@mTiO2@Ag-四环素(FSmTA-T)复合材料用以解决抗生素耐药和纳米Ag团聚、强毒性问题.研究结果显示,制备的复合材料中纳米Ag量子点的粒径约为2.84 nm,可与四环素环3中的羰基键合,同时,相比四环素,复合材料对大肠杆菌、金黄色葡萄球菌、耐四环素沙门氏菌和白色念珠菌均具有较高的抑菌活性,并可有效破坏细菌细胞壁而使其死亡,且对哺乳细胞的毒性降低为原来的1/3.因此,其优越的抑菌活性可应用于污水处理领域.
Abstract
Tetracycline antibiotics are widely used because of their high efficiency,low toxicity and broad-spectrum bacteriostasis,but with the abuse of antibiotics leading to the emergence of a large number of resistant bacteria,the medicinal value of tetracycline antibiotics gradually decreases.Although the ultra-small particle size of Ag can inactivate bacteria and even drug-resistant bacteria,it is highly toxic and easy to agglomerate when used alone.Therefore,in this study,the core-shell mesoporous Fe3O4@SiO2@mTiO2@Ag-tetracycline(FSmTA-T)composite was designed to solve the problems of antibiotic resistance,Ag nanoparticles agglomeration and strong toxicity by using the principle that the d orbital of Ag is a full electron structure and can be coordinated with the electron donor group.The results show that the particle size of the Ag quantum dots in the prepared composite is 2.84 nm,which could be bonded with the carbonyl group in tetracycline ring 3,and compared with tetracycline,the composite material has high bacteriostatic activity against Escherichia coli,Staphylococcus aureus,tetracycline-resistant Salmonella and Candida albicans,and could effectively destroy the bacterial cell wall and make it die,while the toxicity is reduced to 1/3 of the original.Therefore,its superior bacteriostatic activity can be applied to sewage treatment.
关键词
抑菌/纳米复合材料/抑菌机制/四环素/耐药/Ag量子点Key words
bacteriostasis/nanocomposite material/antibacterial mechanism/tetracycline/drug resistance/Ag quantum dots引用本文复制引用
基金项目
秦巴生物资源与生态环境国家重点实验室科研基金(SXS-2105)
陕西省教育厅项目(22JK-0317)
陕西省自然科学基金(2023-JC-QN-0162)
陕西省自然科学基金(2023-YBSF-334)
陕西理工大学基础研究基金(SLGKYXM2208)
出版年
2024
NETL
NSTL
万方数据