Transcriptomic Analysis of Narrow-leaf Trait from Zhangpu Wild Rice
As a critical factor in developing ideal-type rice variety,narrow leaves directly affect photosynthesis leading to the biological yield of rice.The Zhangpu wild rice has an obvious narrow-leaf trait,but the molecular regulation mechanism is still unclear.Using the Zhangpu wild rice as the raw material,a backcross introduced line population with R527 background was constructed.Narrow leaf lines were analyzed using transcriptome technology.The results showed that the blade width and 1000-grain weight of the narrow-leaf lines were decreased significantly,while the blade length and grain size were not changed significantly.The results of transcriptome sequencing showed that 650 differentially expressed genes were found in the narrow-leaf lines,of which 381 were up-regulated and 269 down-regulated.In which,the relative c of the 9 differentially expressed genes using the RT-qPCR was basically consistent with the transcriptome sequencing results.The differentially expressed genes were mainly involved in the metabolic pathways such as plant hormones and DNA damage repair.The differentially expressed genes related WD40 proteins were up-regulated in narrow-leaf lines,while most of differentially expressed genes related glycosyl transferase were down-regulated.It was speculated that the WD40 proteins and glycosyl transferases might synergistically regulate the formation of narrow leaves in Zhangpu wild rice by affecting plant hormones,DNA damage repair or other metabolic pathways.
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