The distribution characteristics of Bursaphelenchus xylophilus in pinus massoniana during autumn and winter
Autumn and winter are important periods for the census of pine wilt disease and the logging of dead pine trees.Exploring the spatial distribution characteristics of pine wood nematodes(PWN,Bursaphelenchus xylophilus)in Pinus massoniana during this period can effectively improve the detection efficiency of PWN.The distribution characteristics of PWN in different parts of P.massoniana in au-tumn and winter were studied by nematode isolation and morphological observation.The results showed that the detection rate and number of B.xylophilus in different parts in autumn were 66.67%~78.00%and 321.83~429.56 per gram respectively,which were significantly higher than those in winter(33.33%~52.67%and 33.00~52.82 per gram).During autumn,the distribution of PWN within the main trunk of P.massoniana is more concentrated in the 8~11 m range,especially at the position 1 meter below the top,where reaches its highest at 1349.50 individuals per gram.This indicated that autumn was a more suitable period for conducting general surveys of pine wilt disease.During this period,the primary lateral branches and the upper part of the main trunk of the P.massoniana were key sampling and testing sites.This facilitates an increase in the detection rate and efficiency of PWN identification,can significantly shorten the time re-quired for detection and identification,and reduce the cost of the investigation.
Bursaphelenchus xylophilusPinus massonianaspatial distributionplant quarantineautumn census