Long-term subculture of embryogenic calli and their genetic variation of thechromosome number in longan
Friable embryogenic calli induced and then selected from the culture of longan (Dimocarpus longanLour. ) immature embryos maintained strong capacity of somatic embryogenesis after having been subculturedfor over 6 years. The cytological examinations showed that there were some cell variations with thechromosome number 2n=3x= 45 or 2n= 4x= 60 or others in some cell clusters of callus lines, which was ofgreat value for obtaining cell variants in vitro in longan.