首页|淡紫拟青霉NH-PL-03菌株的培养特性

淡紫拟青霉NH-PL-03菌株的培养特性

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跟踪检测了淡紫拟青霉NH-PL-03菌株液体培养过程中D(620 nm)值、pH值、菌丝干重、产孢量以及基质消耗(碳、氮)等培养参数,对培养因子进行了相关分析和主成分分析,并对该菌株的培养阶段进行了聚类分析.结果表明:菌丝干重、产孢量、D(620 nm)值分别与营养生长、生殖生长、生理特性主成分;基质消耗(C、N)与产物生成呈显著相关;当λ=2.582时,可将淡紫拟青霉菌株NH-PL-03培养过程分为4个阶段,第1阶段为适应期,培养时间为1-2 d,菌的培养参数变化不大;第2阶段为生长期,培养时间为3-5 d,菌体开始生长,D(620 nm)值上升,pH值下降,菌丝和产孢量增加;第3阶段为对数期,培养时间为6-7 d,D(620 nm)值、pH值上升,菌丝和产孢量急剧增加;8 d后进入稳定期(第4阶段),D(620 nm)值、pH值趋于稳定,菌丝干重和产孢量达到峰值并趋于稳定.
Culture characteristics of Paecilomyces lilacinus (Thom.) Samson strain NH-PL-03
Growing indexes of Paecilomyces lilacinus (Thom. ) Samson strain NH-PL-03 such as D(620 nm) value, pH value, dry weight of mycelia, sporulation and the C, N consuming in the liquid culturing process had been systemically investigated. The results were as follows; (1) the rhythm growing were interrelated to C and N consuming significantly. (2) dry weight of mycelia, sporulation and D(620 nm) value were the principal components of nutritious growth, reproduction and physiological status, respectively. (3) based on the cluster analysis, when λ was equal to 2.582 the cultural process can be divided into four stages: the first one (1 -2 d) was an adaptation stage, with all growing indexes gradually changing; the second one(3 - 5 d) was a growth stage, all growing indexes gained in rapidly, biomass increased significantly; the third one (6-7 d) was a logarithmic stage, all growing indexes leaped forward and the biomass increased rapidly; the fourth one (after 8 d) was a stagnant stage, all growing index was stable relatively.

Paecilomyces lilacinusdry weight of myceliasporulationC, N consumingcluster analysis

李芳、刘波、黄素芳、史怀、刘芳、陈家骅

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福建农林大学植保学院,福建,福州,350002

福建省农科院生物技术中心,福建,福州,350003

淡紫拟青霉 菌丝干重 产孢量 基质消耗 聚类分析

国家高技术研究发展计划(863计划)

2002AA244031-2

2005

福建农林大学学报(自然科学版)
福建农林大学

福建农林大学学报(自然科学版)

CSCD北大核心
影响因子:0.582
ISSN:1671-5470
年,卷(期):2005.34(1)
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