Cloning and sequence analysis of APETALA1 homologous gene from cultivated and wild loquat
A pair of primers were designed according to the conservative regions of the plant floral meristem identity gene-APETALA1 (AP1 ) homologous genes. In both loquat cultivar Xiangzhong 11 and wild variety Liye, a fragment about 350 bp of AP1 homologous gene was amplified respectively by polymerase chain reaction ( PCR ) using the genomic DNA. The fragments were cloned into pUCm-T vector, and then sequenced. The results showed that a fragment of AP1 homologous gene was obtained: the fragments from the two varieties were named with ejAP1 and epAP1 accordingly. The results of sequence analysis indicated that epAP1 and e/AP1 had little difference, there were two introns in the fragment of both varieties of loquat, and the exons were most similar with each other, there was a difference in one nucleotide acid between epAP1 and ejAP1. They encoded 36 amino acids. The two genes were registered in GenBank with the the accession numbers of AY549306, AY571786 accordingly. After the deduced amino acid sequences of the fragments were submitted to GenBank and blasted with AP1 homologous genes of other plants, it was found that the homology with most of the other AP1 homologous genes reached 80% , especially to Malus family-apple, the homology reached 91% (cultivar) and 94% (wild) , to the highest level. This result suggested that ejAP1 and epAP1 genes might have the same function as other API homologous genes.
国家科技期刊平台
NSTL
万方数据
维普
