首页|IGF2基因多态性检测与生长性状相关性分析

IGF2基因多态性检测与生长性状相关性分析

扫码查看
采集395头核心群的种猪耳样,采用PCR-SNaPshot方法检测长白猪和大白猪胰岛素样生长因子2(IGF2)基因内含子3的位点多态性,并分析各基因型与生长性状的相关性.结果表明,长白和大白猪中均存在3种基因型,大白猪3种基因型分布为:AA>GA>GG,长白猪3种基因型分布为:GA >AA>GG.大白和长白猪的A等位基因频率分别为0.710、0.541,G等位基因频率分别为0.290、0.459.AA基因型个体比GA和GG基因型个体生长速度快,等位基因A对猪的生长具有正效应,同时背膘厚变薄.3种基因型个体在达100 kg活体背膘厚及其估计育种值(EBV)的大小为:GG>GA>AA.因此,在种猪选育过程中,逐代选择带有A等位基因的个体提高后代的生长速度,降低背膘厚,加快种猪的选育进展,建立IGF2有利基因型纯系种猪核心群.
The polymorphism detection of IGF2 gene and correlation analysis with growth traits
The polymorphism at intro 3 of IGF2 gene in Landrace and Yorkshire was detected by PCR-SNaPshot in 395 pig ear skins of nucleus group, and the correlation between the genotypes of IGF2 gene and growth traits was analyzed. The result indicated that there were three kinds of genotypes in the two swine populations. The three kinds of genotypes frequencies of Yorkshire pigs were AA > GA > CG, and those of Landrace were GA > AA > GG. The A allele frequencies of Yorkshire and Landrace pigs were 0.710 and 0.541 respectively, G allele frequencies were 0.290 and 0.459 respectively. Correlation analysis result showed that the AA genotype had faster growth rate than GA and GG genotypes. The allele A had a positive effect on growth rate, and led to backfat thickness. The living mean BFT to 100 kg and the estimated breeding value (EBV) of the living mean BFT to 100 kg of pig with the three kinds of genotypes was as follows; GG > GA > AA. Therefore, in the selection process of pig breeding, the individual with allele A should be selected generation-by-generation for raising the growth rate, reducing the backfat thickness and speeding up the progress of pig breeding, and finally a favorable genotype of IGF2 pure line core group was established.

piginsulin-like growth factor 2 (IGF2)polymorphism detectiongrowth traitscorrelation analysis

林长光、刘亚轩、吴悌霖、林金玉、郭长明、朱志明

展开 >

福建光华百斯特生态农牧发展有限公司,福建尤溪365106

福建省农业科学院畜牧兽医研究所,福建福州350013

三明市畜牧兽医水产局畜牧站,福建三明365000

胰岛素样生长因子2(IGF2) 多态性检测 生长性状 相关性分析

福建省发展和改革委员会资助项目国家生猪产业技术体系建设专项福建省生猪严业技术体系建设项目

闽发改高技[2010]299号CARS-36闽农科教[2009]200号

2011

福建农林大学学报(自然科学版)
福建农林大学

福建农林大学学报(自然科学版)

CSTPCDCSCD北大核心
影响因子:0.582
ISSN:1671-5470
年,卷(期):2011.40(6)
  • 5
  • 10