Phylogenetic analysis of bivalve based on mitochondrial ND1,COX1 and Cytb genes
In order to investigate the phylogenetic relationships among main groups of bivalve molluscs, mitochondrial genomic ND1,COX1 and Cytb sequences of the Chinese population of Scapharca broughtonii were determined by PCR amplification and se-quencing. The homologous sequences of 56 species,which belong to 11 orders and 20 families, were selected from Genbank, and the phylogenetic tree was constructed by Neighbor-Joining(NJ) method and maximum likelihood(ML) method. The mitochondrial gene variation and the evolutionary relationship of the molecular system were studied. The results showed that many indels (inser-tions/deletions) of the ND1,COX1 and Cytb sequences of bivalve molluscs were observed, so there is polymorphism in sequence length of these sequences. The A+T content was significantly higher than that of G+C,indicating an obvious AT preference. Genetic distances among families and orders were from 0.169 to 0.468 and 0.323 to 2.557,respectively.NJ and ML trees showed that all bi-valve species formed a monophyletic branch. The phylogenetic relationship of Pteriomorphia was different in the phylogenetic tree constructed by different genes. The Heterodonta was clustered as a monophyletic glade, and species of Veneroida and Myoida were not completely gathered to their branches,but nested with each other.Species of the family Hiatellidae were separated from the order Myoida. The Solemyoida (Cryptodonta) located at the base of the phylogenetic tree,and the Palaeotaxodonta and Palaeoheterodonta were clustered together,implying that they may have a close genetic relationship.
国家科技期刊平台
NSTL
万方数据
维普
