[Objective]Few researches were focused on the mitochondrial genome of Prunus despite its numerous and complex mem-bers.In this paper,comparative genomics was performed on the mitochondrial genome of Prunus to provide reference for their phy-logeny and population identification.[Method]Based on the mitochondrial genome sequences of 7 Prunus species published in NCBI database,their characteristics,composition,gene transfer,genetic variation and phylogeny were analyzed by bioinformatics meth-ods.[Result]The mitochondrial genome of the 7 Prunus species ranged from 422 215(P.kanzakura)to 535 727 bp(P.mume)in length,and they had an average GC content of about 45％and shared similar numbers of coding sequence.Transmembrane domain analysis showed that some open reading frames may contain signal peptides,and analysis on gene transfer from chloroplast to mito-chondria suggested that the tRNA genes were more conserved than the rRNA genes.The mitochondrial genome codon of the 7 Prunus species showed weak codon bias,but had strong collinearity,and most of the protein-coding genes were under purification selection during evolution.Mitochondrial genome and single-gene phylogenetic analysis showed closer relationships between P.kanzakura and P.schneiderana,P.mume and P.sibirica,as well as P.salicina and P.mira,which were consistent with the botanical classification standard.[Conclusion]As an important part of plant whole genome,the mitochondrial genome of Prunus has relatively conserved se-quence,and can be used for species identification,phylogeny and etc.
万方数据
维普
