Effect of aquacultural reclamation on community structure of soil iron-reducing bacteria in coastal wetlands
[Objective]The study aimed to explore the structural changes,functional evolution and influencing factors of soil iron-re-ducing bacteria community,to understand the changes of wetland microbial diversity before and after aquacultural reclamation.[Method]Three typical wetlands in China's coastal regions,including Suaeda salsa(the dominant vegetation)wetland of Yellow River Estuary in Shandong Province,Aegiceras coniculatum wetland of Jiulong River Estuary in Fujian Province,and Bruguiera gymnorrhiza wetland of Dongzhai Port in Hainan Province were set up as the experimental sites,where the community structures of soil iron-reducing bacteria were analyzed via high-throughput technology,and the contents of soil ferric Fe(Ⅲ)and ferrous Fe(Ⅱ)irons were determined by hydrochloric acid-phenanthroline colorimetric method.[Result]After aquacultural acclamation,Bacte-roidetes was the dominant phylum in the S.salsa wetland of Yellow River Estuary,while it was replaced by Proteobacteria in the oth-er 2 wetlands;at the genus level,Yellow River Estuary was dominated by Bacteroides solely,while Jiulong River Estuary was domi-nated by Vibrio,Desulfuromonas and Thiobacillus,and Dongzhai Port was dominated by Aeromonas and Thiobacillus.(2)After aq-uacultural reclamation,the relative abundances of bacterial chemoheterotrophy and fermentation were increased in the Yellow River Estuary,while Dongzhai Port had greater relative abundances in functions relating to chemoheterotrophy,aerobic-chemoheterotro-phy,fermentation and nitrate-reduction,and Jiulong River Estuary showed higher relative abundances in aerobic-chemoheterotrophy and nitrate-reduction.(3)According to redundancy analysis,soil pH had the greatest impact on the community structure of iron-re-ducing bacteria comparing to other environmental factors,and Fe(Ⅲ)had greater impacts than Fe(Ⅱ)on the community structure of iron-reducing bacteria.[Conclusion]After aquacultural reclamation,the physiochemical properties of soil,and contents of Fe(Ⅲ)and Fe(Ⅱ)all exerted various impacts on community structure of iron-reducing bacteria,especially pH;when natural wet-lands were reclaimed for aquacultural purpose,the community structure of iron-reducing bacteria tended to change favorably for aq-uaculture,showing the adaptability of microorganisms.
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