Correlation analysis between drug and zinc resistance of porcine Enterococcus
[Objective]The porcine Enterococcus resistance to heavy metal zinc and drug,its epidemiological feature and potential cor-relation between zinc and drug resistance genes were analyzed to provide reference for the use of antimicrobial drugs and ZnO in breed-ing.[Method]Enterococcus were isolated from 395 pig stool samples with specific selection medium,and their sensitivity to 9 antimi-crobial drugs and ZnSO4 were determined by microdilution method.Meanwhile,the presence of drug and zinc resistance genes were i-dentified through PCR.[Result]A total of 348 Enterococcus isolates were obtained,with an isolation rate of 88.10% .The drug suscep-tibility testing of Enterococcus isolates showed the highest resistance to erythromycin at 96.81% and the lowest to vancomycin at only 0.71% .Multidrug resistance analysis revealed that Enterococcus isolates with quadruple resistance accounted for the highest proportion(31.91% ).The ZnSO4 sensitivity testing indicated that 52.13% of the isolates had a minimum inhibitory concentration(MIC)≥12 mmol·L-1.The zinc resistance gene zntA had the detection rate of 37.93% .The detection rate of gyrA gene encoding the A subunit of DNA gyrase was 92.79%,while that of macrolide resistance gene mefA was only 0.31% .Correlation analysis indicated that zinc resist-ance gene was significantly positively correlated(P＜0.05)with the tetS gene encoding ribosome protection protein and tetracycline ef-flux gene tetL,but negatively correlated with the tetracycline efflux gene tetG(P＜0.05),gyrA and rRNA methylase gene ermB.[Con-clusion]The heavy metal zinc may induce the development of resistance and multidrug-resistant phenotypes in enteric Enterococcus of swine,and antimicrobial drugs may also influence the zinc resistance of Enterococcus in pig feces.
万方数据
维普
