Effect of acidic diets on Nosema ceranae reproduction and immunity of Apis mellifera ligustica
[Objective]The impact of acidic diet on Nosema ceranae reproduction and immunity of Apis mellifera ligustica were investi-gated to verify if acidic diets can prevent Nosemosis and provide scientific basis for the rational use of acidic diet in honeybees.[Meth-od]Four experimental groups with 100 bees per group were set up,including N.ceranae infection group,infection group with citric acid diet(citric acid content is 50 g·L-1),infection group with acetic acid diet(acetic acid content was 400 mL·L-1),and control group without infection.The expression levels of immune genes(Apidacin,Abaecin,Defensin2,Defensin1,Dorsalk2,Demeless,Dredd,Hymenoptaecin,Lysz,PGRpsc4300 and Toll)were detected at 1 and 3 d post inoculation.Additionally,the cumulative survival was analyzed at 7 d post inoculation,and spore load was quantified in the remaining bees.[Result]There were significant differences in survival rates among the 4 groups(P＜0.001),and the mortality of the infection group with citric acid diet was significantly higher than that of the infection group(P＜0.05).The spore load in the midgut of honeybees were not significantly different between the infec-tion groups with and without acidic diets(P＞0.05).The relative expression of the 11 immune genes were no statistically decreased at 3 d post inoculation(P＞0.05),but the gene[PGRPSC4300(P＜0.05)and Abaecin(P＜0.05)]were down-regulated in group with a-cidic diet.[Conclusion]Citric acid(50 g·L-1)and acetic acid(400 mL·L-1)diet can neither limit N.ceranae reproduction nor promote bee immune responses,implying their ineffectiveness on treating N.ceranae infection.
万方数据
维普
