Effect of Cenchrus fungigraminus on soil enzyme activity,microbial community structure and functional diversity of Ningxia moderate saline-alkali land
[Objective]The microbial community structure and diversity of the rhizosphere soils planted with Cenchrus fungigraminus were studied to provide a basis for the improvement of moderate saline-alkali lands in Ningxia Hui Autonomous Region.[Method]Soil enzyme activities were determined by trace method,and the community structure and functional diversity of rhizosphere bacteria and fungi were measured by next generation sequencing method.[Result]After planted with C.fungigraminus,the volumetric weight,salt content and alkali-hydrolyzable nitrogen content of the remediated soils were down by 8.96%,71.90% and 56.20%,re-spectively,while the contents of soil organic matter(SOC),available phosphorus(AP)and available potassium were up by 35.25%,81.97% and 2.51%,respectively.The remediated soils also saw greater alkaline phosphatase and sucrase activities by 16.87% and 6.14%,respectively.In the meantime,the remediated soils varied significantly in soil fungal Simpson index and Shan-non index after planted with C.fungigraminus.In terms of the community structure,the remediated soils had greater relative abun-dances in Chloroflexi,Myxomycota,Verrucomicrobia,Basidiomycota,Mortierellomycota,Blastocladiomycota,Mucoromycota,Ol-pidium and Rozellomycota;and they also had greater operational taxonomic units(OTU)number of bacteria(up by 0.74% ),and lower OTU of fungus(down by 42.24% )compared with the control.Moreover,clustering analysis showed that there were significant differences on the bacteria and fungus at the phylum and genus level between the experimental and control groups,and redundancy analysis further revealed SOC,soil alkaline phosphatase activity,AP and water-soluble salts were the major environmental factors that altering the properties of soils after planted with C.fungigraminus.[Conclusion]Planting C.fungigraminus contributed to higher soil enzyme activity,richer microbial diversity,and better soil microbial community environment in moderate saline-alkali land.
万方数据
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