Biomass,nutrient uptake capacity and enzyme activities of six species of ectomycorrhizal fungi from different areas
[Objective]The study aimed to understand the mechanisms of ectomycorrhizal fungi(ECMF)in advancing tree nutrient,forest health,soil remediation,and their own environment adaptability,which was contributed to the screening,research and pro-duction application of ECMF.[Method]Six fungal isolates,including Boletus sp.,Cenococcum geophilum,Lactarius deliciosus,Pisolithus tinctorius 715,Russula delica,and Suillus grevillei,originated from various forests across China,were grown in Pachlews-ki liquid culture medium(glucose as the carbon source),and then the growth rate,nutrient uptake ability,and type and activity of intracellular and extracellular enzymes were analyzed.[Result]ECMF varied greatly in the biomass,nutrient uptake and enzyme ac-tivity in mycelia and culture medium.R.delica had the highest biomass(160.93 mg),and C.geophilum had the minimal biomass(23.73 mg).The mycelial nutrient contents based on dry weight were as follows:nitrogen(N)2.12-11.23 mg·g-1,phosphorus(P)2.43-16.99 mg·g-1,and potassium(K)3.67-17.29 mg·g-1.L.deliciosus,Boletus sp.,and R.delica accumulated the high-est amounts of N,P,and K respectively in their mycelia,implying the differences in nutrient uptake capacity in response to fungal strains after root-ectomycorrhizal fungus associations were established.In addition,endoenzymes of dehydrogenase,acid phospha-tase,urease,catalase,chitinase and proteinase were found in all fungal mycelia;apart from dehydrogenase,other five enzymes were also detected in liquid culture medium with fungal inoculation.In terms of endoenzyme enzymes,the acidic phosphatase(2.54 U·g-1)and pyrophosphatase(4.46 U·g-1)activities of S.grevillea were significantly higher than those of the other ECMF strains;the dehydrogenase(11.06 U·g-1)and cellulase(118.44 U·g-1)activities of P.tinctorius 715 were the highest among all the fun-gal strains;C.geophilum and Boletus sp.had the highest catalase(11.32 U·g-1)and protease activity(162.51 U·g-1),which were significantly higher than those of the others.In analysis of exoenzyme activity,among strains,R.delica,P.tinctorius 715,and C.geophilum had significantly higher activities of acid phosphatase(16.79 U·mL-1),chitinase(85.18 U·mL-1),and catalase(0.15 U·mL-1),respectively;urease activity in northern strains(C.geophilum and S.grevillea)was significantly higher than that in southern strains(Boletus sp.,L.deliciosus,P.tinctorius 715,and R.delica);the protease activity of southern strains(1.16-1.71 U·mL-1)was significantly higher than that of northern strains(0.28 U·mL-1 and 0.72 U·mL-1).[Conclusion]There are big differences in the extracellular enzymes activities among ECMF strains,which implies a selective utilization of organic components,and a key reason for their wide distribution in various types of forest soil.
万方数据
维普
