1株野生高效纤维素降解菌筛选、鉴定其酶活力测定

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中文摘要：为从腐熟的土壤中分离出产纤维素酶的细菌，并进一步研究该菌株的纤维素降解能力。采用羧甲基纤维素(CMC)-刚果红染色法，筛选出1株野生纤维素降解菌ZG2-3，采用16S rRNA分子鉴定其种类，同时从促腐剂中筛选出4株商品纤维素降解菌，比较野生纤维素降解菌与商品纤维素降解菌的酶活差异。结果表明:从娄底市珠山公园桂花树林下腐熟的土壤中采集分离，通过羧甲基纤维素(CMC)-刚果红染色法，筛选得到了纤维素降解能力较高的1株细菌ZG2-3。经鉴定ZG2-3为枯草芽孢杆菌Bacillus subtilis。ZG2-3的羧甲基纤维素酶(CMCase)活力和滤纸酶(FPA)活力分别为0。054 IU·mL-1和0。02 IU·mL-1，其中ZG2-3的CMCase活力显著高于商品纤维素降解菌。

外文标题：Screening and Identification of a Wild Cellulose-degrading Strain with High Efficiency and Determination of Its Enzyme Activity

外文摘要：In order to isolate cellulase-producing bacteria from the overrotten soil and further study the cellulose degradation ability of the strain,a wild cellulose-degrading strain ZG2-3 was screened by using the carboxymethyl cellulose (CMC)-congo red staining method.Then,the 16 S rRNA molecular identification was used to identify the species,and four commercial cellulose-degrading bacteria strains were screened from the promoting-decomposing agent.Last,the enzyme activity differences between the wild cellulose-degrading bacteria and the commercial cellulose-degrading bacteria were compared.The results showed that one strain of bacteria with high cellulose degradation ability,ZG2-3,was screened by using the carboxymethyl cellulose (CMC)-congo red staining method,which was collected and isolated from the overrotten soil under the osmanthus forest in Zhushan Park,Loudi City.ZG2-3 was identified as Bacillus subtilis.The carboxymethyl cellulase (CMCase) activity and filter paper enzyme (FPA) activity of ZG2-3 were 0.054 IU·mL-1 and 0.02 IU·mL-1,respectively,and the CMCase activity of ZG2-3 was significantly higher than that of the commercial cellulose-degrading bacteria.

外文关键词：

CelluloseBacillus subtilisScreeningIdentificationEnzyme activity

作者：

廖凯、肖畑、颜健红、郑慧新、敬媛蓉、刘秀、倪弦之、金晨钟、郭开发

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作者单位：

湖南人文科技学院/有害生物绿色防控重点实验室湖南娄底 417000

娄底市农业农村局湖南娄底 417000

关键词：

纤维素枯草芽孢杆菌筛选鉴定酶活力

基金：

农药无害化应用湖南省普通高等学校科技创新团队(2024年)湖南省区域联合基金项目湖南省区域联合基金项目湖南省教育厅重点项目娄底市2023年度科技创新研发项目(2024年)湖南省研究生创新项目湖南省大学生创新创业训练项目

项目编号：

2023JJ504662024JJ727922A0609CX20231271S202310553003X

出版年：

2024

DOI：

10.13651/j.cnki.fjnykj.2024.05.003

福建农业科技

福建省农业科学院福建省农学会

福建农业科技

影响因子：0.254

ISSN：0253-2301

年,卷(期)：2024.55(5)