高产果糖基转移酶的黑曲霉全细胞固定化条件优化
Optimization of the Conditions for Whole-cell Immobilization of Aspergillus niger with High Yield of Fructosyltransferase
余超凡 1周明炀 1汤淑玲 1吴映姿 1李俊鹏 1吕子键 1何文锦 1郑毅1
作者信息
- 1. 福建师范大学生命科学学院,福建 福州 350117
- 折叠
摘要
采用包埋法固定化高产果糖基转移酶的黑曲霉全细胞,以海藻酸钠-壳聚糖为载体,分别通过单因素和正交试验优化,获得最佳固定化条件.单因素优化试验结果表明:海藻酸钠最适浓度为 2%,菌体包埋量最适为 150 g·L-1,壳聚糖和CaCl2 最适浓度均为 4%.正交试验结果表明:海藻酸钠浓度为 3%,菌体包埋量为 200 g·L-1,壳聚糖浓度 5%,CaCl2 浓度为 3%时,酶活回收率可达到最佳的 74.7%.验证试验表明该固定化细胞连续进行 7 个批次合成低聚果糖的催化,仍有 96.85%的残余活力,具备很好的应用潜力,为合成低聚果糖提供了新的技术路线.
Abstract
The whole cells of Aspergillus niger with high yield of fructosyltransferase were immobilized by using the embedding method.Then,the sodium alginate-chitosan was used as the carrier,and the optimum immobilization conditions were obtained by using the single factor and orthogonal experiment optimization,respectively.The single-factor optimization test results showed that:the optimal concentration of sodium alginate was 2%,the optimal quantity of bacteria embedded was 150 g·L-1,and the optimal concentrations of chitosan and CaCl2 were both 4%.The results of orthogonal test showed that when the concentration of sodium alginate was 3%,the quantity of bacteria embedded was 200 g·L-1,the concentration of chitosan was 5%,and the concentration of CaCl2 was 3%,the recovery of enzyme activity could reach the optimal 74.7%.The validation test showed that the immobilized cells were catalyzed for the synthesis of fructooligosaccharides in seven consecutive batches,and still had 96.85%residual activity,which had good potential for application and provided a new technical route for the synthesis of fructooligosaccharides.
关键词
果糖基转移酶/低聚果糖/黑曲霉/固定化细胞Key words
Fructosyltransferase/Fructooligosaccharides/Aspergillus niger/Immobilized cells引用本文复制引用
基金项目
国家糖料产业技术体系建设专项(CARS-170501)
校大学生创新训练计划(cxxl-2024271)
出版年
2024
国家科技期刊平台
NSTL
万方数据