摘要
为了探究不同类别的植物源性抗氧化剂对太湖猪精液常温保存效果的影响,选取了姜黄素、β-胡萝卜素两种常见的抗氧化剂,分别以不同浓度(姜黄素:0、3、6、9µg·mL-1;β-胡萝卜素:0、10、20、40µg·mL-1)添加到太湖猪精液稀释液中,采用目测法、低渗肿胀法和瑞氏-吉姆萨染色法检测精子在 17℃保存环境中第 1、3、5、7 d的活力、质膜完整率和顶体完整率.结果表明:添加一定浓度的姜黄素、β-胡萝卜素均能显著提高精子质量,其中姜黄素的最适添加浓度为 6µg·mL-1,β-胡萝卜素的最适添加浓度为 20µg·mL-1.
Abstract
In order to explore the effect of different types of phytogenic antioxidants on the preservation of Taihu pig semen at room temperature,the two commonly used antioxidants,curcumin and β-carotene,were selected.They were added at different concentrations(curcumin:0,3,6,9 µg·mL-1;β-carotene:0,10,20,40 µg·mL-1)to the semen diluent of Taihu pig.The sperm vitality,plasma membrane integrity rate and acrosome integrity rate were detected by using the visual observation,hypotonic swelling method and Wright-Giemsa staining method on the 1 st,3 rd,5 th and 7 th day of preservation at 17 °C.The results showed that the addition of certain concentrations of curcumin and β-carotene could significantly improve the sperm quality,with the optimal concentrations being 6µg·mL-1 for curcumin and 20µg·mL-1 for β-carotene.
基金项目
国家自然科学基金项目(32002175)
金陵科技学院2021年科教融合项目(2021KJRH32)
金陵科技学院博士科研启动基金(jitb-202035)
大学生创新创业训练计划(202313573097Y)
国家科技期刊平台
NSTL
万方数据