Study on the Component Analysis of Essential Oil from PK-type Perilla frutescens Leaves by GC-MS and Its Antioxidant Activity
In order to deeply investigate the antioxidant potential of essential oil from PK-type Perilla frutescens leaves,an essential oil with a content of perilla ketone higher than 40%was screened out by using the gas chromatographic analysis(GC)technology.Then,the chemical composition of the essential oil was further identified by using the GC-MS technology,and the antioxidant activity in vitro of the essential oil was analyzed,which provided a scientific basis for the development and utilization of the essential oil from PK-type Perilla frutescens leaves in the field of antioxidation.The results showed that there were 17 kinds of chemical components with the content of more than 0.5%in the essential oil from Perilla frutescens,all of which were terpenes and their derivatives,accounting for 89.72%of the total content of essential oil.The components with higher content were perilla ketone(44.98%),isoegomaketone(13.06%)and β-caryophyllene(10.53%).The experimental results of antioxidant activity in vitro showed that:the essential oil from PK-type Perilla frutescens leaves had scavenging ability to both DPPH and ABTS radicals,with IC50 values of 7.00 and 2.66 mg·mL-1,respectively.It also had certain iron reduction ability and total antioxidant capacity,with the RP0.5 AU values of 11.96 and 1.83 mg·mL-1.It was shown that all the above antioxidant activity indexes of the essential oil from PK-type Perilla frutescens leaves were positively correlated with the concentration.
Essential oil from PK-type Perilla frutescensleavesComponent analysisGC-MSAntioxidant activity
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