Diversity Analysis of Endophytic Bacteria in Kandelia candel Based on 16S rDNA Sequence
The diversity of culturable endophytic bacteria in the roots,branches and leaves of Kandelia candel in the wetland of Minjiang River estuary was analyzed,in order to provide support for the study of endophytic bacteria community structure,distribution,stability and functional endophytic bacteria in Kandelia candel.In this study,the culturable endophytic bacteria were isolated from different tissues of Kandelia candel,and the diversity of endophytic bacterial was analyzed based on 16S rDNA gene sequence alignment and phylogeny.The results showed that 36 strains of culturable endophytic bacteria were isolated from three tissues of Kandelia candel,belonging to 11 genera and 22 species(2 strains among which were not officially named).Bacillus and Vibrio were the dominant genera,accounting for 21.2%and 19.4%of the total strains,respectively.There were differences in the distribution and diversity of endophytic bacteria in different tissues,among which the number of endophytic bacteria was the highest in roots(27 strains,accounting for 75%of the total number of strains),followed by branches(6 strains,accounting for 16.7%of the total number of strains),and the least in leaves(3 strains,accounting for 8.3%of the total number of strains).The endophytic bacteria in Kandelia candel had rich species diversity in the wetland of Minjiang River estuary,and there were differences among different tissues.The diversity of endophytic bacteria in the roots was the most abundant.