Artificial Seedling and Juvenile Shell Cultivation Technology of Paphia amabilis
The techniques of artificial breeding and juvenile shellfish cultivation of Paphia amabilis were discussed in order to provide technical data for large-scale and efficient breeding and production of P.amabilis seedlings.The clustering analysis and single factor analysis of variance were used to study the key techniques of species identification and seedling breeding of P.amabilis.The results showed that the similarity between the COI sequences of the juvenile shellfish and those of P.amabilis was 100%.The measured juvenile shellfish were all P.amabilis.The parent shellfish with an average shell length of 45.37 mm were selected during the breeding period of P.amabilis.The fertilized eggs were obtained by the method of drying in the shade and ammonia seawater stimulation.The induced spawning rate and hatching rate were 59.25%and 91.68%,respectively.Under the conditions of water temperature of 28.1℃and salinity of 27.5,the fertilized eggs developed into the D-shaped larvae after 20 hours,and the larvae were developed to metamorphosis larvae after 9 days with the culturing density of 1-2 inds·mL-1.After 3-5 days of cultivation with fine sand as the attachment base,all the larvae were developed into juvenile shellfish,and the metamorphosis rate could reach more than 50%.In the early stage,the juvenile shellfish were cultured with sand,while the juvenile shellfish with the shell length greater than 1 mm were cultured without sand.The shell length of juvenile shellfish could reach more than 12 mm after 6 months of indoor cement pond cultivation.The juvenile shellfish with the shell length greater than 12 mm were selected to carry out the seedling cultivation experiment in outdoor cement pond.The results showed that the growth rate and survival rate of the overhead group were significantly higher than those of the non-overhead group.
Paphia amabilisArtificial seedlingLarvae cultivationCultivation of juvenile shellfish
万方数据
维普
