Establishment of the Mouse Model of Klebsiella pneumoniae Liver Abscess
In order to construct a stable and reliable animal model of Klebsiella pneumoniae liver abscess(KPLA),about 18 g healthy ICR mice were selected,and the highly virulent animal-derived Klebsiella pneumoniae clinical isolated strain KP001 was used as the model strain.Then,the inoculation methods(including the gastric irrigation,nasal drip and intraperitoneal injection,with the number of inoculated bacteria was 1×107 CFU)and the inoculation dosage were explored.The biochemical indexes of serum samples from the mice with successful modeling were determined,including the alanine aminotransferase,aspartate aminotransferase,alkaline phosphatase,γ-glutamyl transferase,albumin,lactate dehydrogenase,and total bilirubin.The results showed that the survival rate of mice treated with gastric irrigation was 100%,but there was no abscess in the liver.The mortality rate of mice treated with nasal dripping was 100%,with obvious lung lesions,and the liver abscess occurred in 40%of the dead mice.After the intraperitoneal injection of 1×106 CFU bacteria,the survival rate of mice was more than 75%,and the liver abscesses appeared in the surviving mice,with the modeling rate of 100%.The pathological sections showed that the liver cells of the successfully modeled mice arranged in disorder,and a large number of inflammatory cells infiltrated around the central vein.The detection of biochemical indicators showed that compared with the control mice,the γ-glutamyl transferase in the serum samples of the successfully modeled mice was significantly increased(P＜0.01),the alkaline phosphatase was significantly decreased(P＜0.01),and the alanine aminotransferase and albumin were significantly decreased(P＜0.05).In conclusion,the analysis showed that the KPLA mouse model could be successfully construct-ed by the intraperitoneal injection of 1×106 CFU KP001 strain.The biochemical indexes such as γ-glutamyl transferase and alkaline phosphatase could be used to evaluate the effect of model establishment.
万方数据
维普
