Mechanism of Lactobacillin PlnJ(G5)on Alleviating the Intestinal Epithelial Cells and Intestinal Inflammation in Mice Induced by LPS
In order to explore the mechanism of lactobacillus PlnJ(G5)modified and preserved in the laboratory on the intestinal epithelial cells IPEC-J2 and the intestinal inflammation in mice induced by lipopolysaccharide LPS,the intestinal epithelial cell IPEC-J2 and model animal mice were selected as the research carriers.LPS was used to establish an inflammatory model,and PlnJ(G5)was added for the treatment.Then,the cell viability was detected by cck-8 and the expression changes of inflammatory cytokines was detected by the real-time fluorescence quantitative PCR.The results showed that 5.0 μg·mL-1 LPS had little effect on the cell viability of IPEC-J2,and the mRNA expression levels of IL-1,IL-6,TNF-α,IFN-α and IFN-β in IPEC-J2 cells treated with this concentration were significantly higher than those in the blank control group,which proved that the inflammatory model of IPEC-J2 cells induced by LPS was successful.The results of CCK-8 detection showed that the safe additive dosage of PlnJ(G5)for IPEC-J2 cells was 20.0 μg·mL-1.The results of qPCR detection showed that after IPEC-J2 cells treated with 5.0 μg·mL-1 LPS for 4 h,the addition of 20.0 μg·mL-1 unheated PlnJ(G5)had the best inhibitory effect on the mRNA expression of IL-1,IL-6,TNF-α,IFN-α and IFN-β.The experimental results showed that PlnJ(G5),which was not heat treated,had the best anti-inflammatory effect on mice induced by LPS.
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维普
