This paper focused on the concentration of polyunsaturated fatty acids(PUFA)in ethyl esters(EE)converted from winterized Schizochytrium sp.oil via urea inclusion method.The impact of various post-treatment methods,cooling temperatures,cooling times,ethanol concentrations,urea/EE ratios,and ethanol/urea ratios,on the urea inclusion process was investigated through one-way tests,and orthogonal tests were conducted to determine the optimal process conditions.The results demonstrated that the utilization of the optimized post-treatment method significantly enhanced the involvement of urea in the inclusion process with EE,resulting in an increased PUFA content in the non-urea included frac-tions.Under the optimized conditions,the contents of docosahexaenoic acid and docosapentaenoic acid in the EE-type microalgal oil were improved from 47.17％and 13.93％to 73.20％and 19.87％,respec-tively.
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