Extraction of Bacterial Genomic DNA Based on CTAB Modified Attapulgite
Attapulgite was modified with HCl and CTAB to increase functional groups on the surface of attapulgite,and the adsorption and de-sorption of modified materials on DNA were studied.X-ray diffractometer(XRD),Fourier transform infrared spectroscopy(FTIR)and scanning elec-tron microscope(SEM)were used to characterize attapulgite before and after modification.The amount,purity and integrity of nucleic acid extraction were investigated with Staphylococcus aureus as the object of nucleic acid extraction.The results showed that the saturated adsorption capacity of the modified material for DNA could reach 18.657 μg/mg,the nucleic acid extraction capacity of Staphylococcus aureus was(5.45±0.46)ng/105 CFU,the structure was intact and the purity was high.
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