The experiment analyzed the effects of two different antibiotic substitution combinations on the growth performance and di-arrhea rate of weaned piglets by adding two different antibiotic substitution combinations to the diet of weaned piglets.The experiment selected 90 ternary hybrid(Duroc×landrace×Yorkshire)healthy weaned piglets with similar average body weight,using a single-factor experimental design,the experimental animals were randomly divided into 3 treatment groups with 3 replicates in each group,and each replicate was 10 piglets.The diet treatment included corn-soybean meal basic diet(control group I),experimental group Ⅱ and experimental group Ⅲ.The experimental period was 35 days.The results showed that the average final weight,average daily gain and feed-to-weight ratio of experimental groups Ⅱ and Ⅲ were significantly higher than those of control group I(P<0.05),and the average daily gain increased by 13.51%and 15.74%,respectively,while the growth performance of weaned piglets in experimental groups Ⅱ and Ⅲ was not significant(P>0.05);in terms of diarrhea rates,the two experimental groups of experimental groups Ⅱ and experimental group Ⅲ were significantly lower than those of control group I,and the difference was significant(P<0.05);the difference in diarrhea rates between experimental groups Ⅱ and Ⅲ was not significant(P>0.05).It can be seen that the combination of plant essential oils,medium-short chain fatty acids and organic acids and antimicrobial peptides,medium-short chain fatty acids and organic acids can improve the growth performance of piglets and reduce the diarrhea rate of weaned piglets.