Establishment and application of a chromatographic immunization method for Corynebacterium kutscheri
In this study,the prepared monoclonal antibody was identified,and based on this,an immunochromatographic assay for Corynebacterium kutscheri was constructed,and a test strip was prepared.The experimental results showed that the monoclonal anti-body obtained by the test had high titer(1.6×103-1.28×104),good specificity and sensitivity.The minimum detection limit of im-munochromatographic colloidal gold strip is 10-6CFU/mL,and there is no cross reaction with Escherichia coli,Salmonella,etc.,which has certain clinical significance.
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