三种口蹄疫病毒O型、A型抗体ELISA检测试剂盒的比较分析
Comparative analysis on three foot-and-mouth disease virus type O and A antibody ELISA detection kits
林雅书1
作者信息
- 1. 柘荣县乡村振兴服务中心 福建柘荣 355300
- 折叠
摘要
目的:比较不同的口蹄疫病毒O型、A型抗体ELISA检测试剂盒的性能差异,为基层兽医实验室选择合适的试剂盒提供参考.方法:采集 174 份已免疫的猪、羊血清样品,选用 3 个不同厂家的口蹄疫病毒O型、A型抗体ELISA检测试剂盒进行抗体检测,比较各试剂盒的抗体阳性率、符合率和便捷性.结果:3 种试剂盒的口蹄疫病毒O型抗体检测阳性率均在 90%以上,符合率为 89.66%;3 种试剂盒的口蹄疫病毒A型抗体检测阳性率为 56.90%95.98%,差别较大,符合率仅 52.87%.结论:3 种口蹄疫病毒O型、A型抗体ELISA检测试剂盒的检测结果存在一定差异,且差异具有统计学意义(P＜0.05);3 种试剂盒均适用于口蹄疫病毒O型抗体的定性测定,不全适用于口蹄疫病毒A型抗体的定性测定.
Abstract
Objective:Compare the performance differences of different Foot-and-mouth disease virus(FMDV)type O and A antibody ELISA detection kits,provide reference to the grassroots veterinary laboratories in selecting a suitable kit.Methods:174 immunized serum samples were collected from pig and goat,and the antibodies were detected by FMDV-O and A antibody ELISA detection kits with three different manufactures,then compared the antibody positive rate,coincidence rate,and convenience of various kits.Results:The FMDV-O antibody positive rate of three kits were all above 90%,and the coincidence rate was 89.66%;the FMDV-A antibody positive rate had a big difference among three kits with 56.90%to 95.98%,and the coincidence rate was only 52.87%.Conclusions:There were certain differences in the detection results of three FMDV-O and A antibody ELISA detection kits,and the differences had statistically significant(P<0.05);all of the three kits could be used for qualitative determination of FMDV-O antibody,but not all apply to FMDV-A.
关键词
口蹄疫/ELISA/抗体检测/试剂盒Key words
Foot-and-mouth disease(FMD)/ELISA/Antibody detection/Kit引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据