Construction of prokaryotic expression vector of porcine epidemic diarrhea virus E gene
Objective:By constructing the prokaryotic expression vector pET-E of porcine epidemic diarrhea virus E gene,it lays a foundation for further research on the prokaryotic expression and function of E gene.Methods:The PEDV E gene was amplified by PCR.After the PCR product was detected by gel electrophoresis,the target fragment was purified and recovered by the kit.After the gel recovered product was ligated with the expression vector pET-32a,it was transformed into Trans BL21(DE3)competent state.In the cells,a single colony was picked and the culture was shaken overnight to extract a plasmid and the positive vector was identified by double digestion.Result:The target fragment of 231 bp was successfully amplified,and the prokaryotic expression vector of PEDV E gene was constructed.Conclusion:In this study,the prokaryotic expression vector of PEDV E gene was successfully constructed,which laid the foundation for further prokaryotic expression of E gene and further research on the function of PEDV E gene.
Porcine epidemic diarrhea virusE geneConstruction of expression vector
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