Establishment and application of an indirect ELISA method for swine fever antibodies based on the A-D antigen region of E2 protein
In this study,the main antigen region E2 A-D of classical swine fever E2 protein was used as the coating antigen to estab-lish an indirect ELISA antibody detection method for swine fever.The concentration of antigen coating was 0.75 μg/mL and the dilu-tion ratio of serum was 1:150.A critical value test was conducted on 123 serum samples that were negative in the rabbit neutralization test,and it was determined that OD450nm≥0.38 was positive,OD450nm≤0.31 was negative,and 0.31<OD450nm<0.38 was suspicious.The specificity,repeatability and sensitivity of the method were tested and the detection ability of this method was compared with the rabbit neutralization test.The results showed that this study could provide an alternative,specific and stable serological detection method for CSF antibody.
Classical swine fever virusIndirect ELISAE2 protein
国家科技期刊平台
NSTL
万方数据
